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Nucleases, also called nucleodepolymerase or polynucleotidase are enzymes able to cut off the phosphodiester bonds that join the adjacent nucleotides in a nucleic acid like RNA and DNA. Nucleases are highly essential cellular machinery that is naturally present in biological…
Complementary DNA or cDNA is a complementary copy of a stretch of DNA produced by recombinant DNA technology. Complementary DNA is a type of DNA that is created by the enzyme reverse transcriptase. Reverse transcriptase is an enzyme that can…
Cosmids are hybrid plasmids that contain the cos sequence of a Lambda phage (bacteriophage). Structurally these are DNA hybrids of plasmids and a lambda phage. Plasmids refer to small circular DNA in the cytoplasm of bacteria or protozoans. These genetic structures…
An ORF or the open reading frame is a continuous stretch of codons in mRNA that contain a start codon (usually AUG) and a stop codon (usually UAA, UAG or UGA). OR A reading frame is a stretch of DNA which…
DNA (deoxyribonucleic acid) is the long polymeric macromolecule of nucleotides containing codes inscribed in the form of biochemical molecules which serve as both the developmental and hereditary unit of living organisms. ScienceDaily defines DNA (Deoxyribonucleic acid) as a nucleic acid…